BIOLOGICAL SYNTHESIS OF TRIANGULAR GOLD NANOPRISMS PDF

Kagajinn However, there is limited information available regarding the synthesis of GNPs using fungus. The strain was subcultured on potato dextrose agar PDA. In recent years the synthesis of nxnoprisms gold nanoparticles is one of the most active triwngular of research in the field of nanotechnology because of vold applications in a number of areas like bio-imaging, bio-medicine, bio-sensors, bio-labels, catalysis and others []. In our study, GNPs formed a single population in the size range of 10 — 40 nm. There are other reports which showed the intracellular synthesis of spherical GNPs in live Alfaalfa plants [12], truncated triangular and spherical GNPs from sun dried Cinamonum camphora leaf extract [13], bio,ogical gold nanoprism from lemon grass extract [14], hexagonal, prism and spherical shaped GNPs using plant surfactants [15], triangular gold nano plates using algal solutions of Chlorella vulgaris [16] and Sargassum sp. For FTIR spectrum analysis the GNPs were centrifuged at 10, rpm for 10 minutes to remove free proteins or other compounds present in the solution.

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Zolokus Biological synthesis of triangular gold nanoprisms. By conventional chemical and physical methods nanocubes [22], nanorods [23], nanowires [24], nanoplates [25] can be synthesized but that require high environmental cost.

Free amine, carboxyl or phosphate groups present in the protein can interact with gold nanoparticles [32,37] and possibly involved in stabilization of GNPs.

AFM was conducted to record the morphological changes of fungal mycelia following GNP synthesis on their surface. The selected area electron diffraction pattern SAED of gold nanoprism Figure 4 B indicated that the entire nanoparticle was nanocrystalline in nature [31]. There are other reports which showed the intracellular synthesis of spherical GNPs in live Alfaalfa plants [12], truncated triangular and spherical GNPs from sun dried Cinamonum camphora leaf extract [13], triangular gold nanoprism from lemon grass extract [14], hexagonal, prism and spherical shaped GNPs using plant surfactants [15], triangular gold nano plates using algal solutions of Chlorella vulgaris [16] and Sargassum sp.

GNPs size was found to be in the range of 10 — 40 nm with maximum population centered on 20 nm Figure 2. In all these respects biological approach would become a better choice for the synthesis of gold nanoparticles GNPs. The synthesis of nanoparticles using biological approaches offers several advantages than chemical synthesis like environmentally acceptable solvent, eco friendly reducing and capping agents. It was quite exciting for us to find this bioprocess where mycelial biomass suspended in double distilled water in presence of chloroauric acid could produce GNPs on their surfaces.

The zeta potential of GNPs was measured to know their colloidal stability and the nature of the charge carried in their surface. The physical nature of GNPs indicated that most of the nanoparticles were prism shaped. Recently cetyltrimethylammonium bromide CTAB has been shown to induce the formation of gold nanorods, triangular prism and spheres through seed mediated approaches depending on iodide contamination present in CTAB which act as shape directing agent [35].

Synthesis of spherical gold nanoparticles GNPs have been reported using the fungus Fusarium oxysporum and Verticillium sp. The selected area electron diffraction pattern SAED of gold nanoprism Figure 4 B indicated that the entire nanoparticle was nanocrystalline in nature So it can be assumed that a micro stress was developed due to the formation of anisotropic nanoprism. In addition gold nanoprism offers several advantages in biomedical applications.

Development of strain in crystals of gold nanoprism was observed from the analysis of crystals. FTIR analysis also showed that protein molecules were involved in stabilization. The GNPs then resuspended in double distilled water and again centrifuged. The Scherrer ring patterns indicate the fcc gold which is nanocrystalline in nature.

All chemical reagents were purchased from Sigma St. A higher magnification image of gold nanoprism is shown in Figure C. B A selected area electron diffraction pattern of GNPs. Gold nanoparticles GNPs synthesized on cell surface were isolated by placing the mycelial suspension on a bath sonicator and sonicated for 15 minutes. The purple colour of Penicillium citrinum biomass is due to the interaction of GNPs with light [21].

Therefore a growing demand has been accumulated to develop environment friendly approaches to synthesize nanomaterials Green Chemistry.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The process was repeated for three times and finally the centrifuged part containing GNPs were redispersed in double distilled water and subjected to FTIR spectroscopy. Although some rod shaped, triangular, cubical, very small circular 3 — 4 nm diameter and rectangular GNPs were present in smaller amounts Figures 3 A and B.

Received January 16 th; revised February 23 rd; accepted April 5 th However, bio application of large nano particles greater than 50 nm are limited due to reduced endocytosis and poor renal clearance [30]. Synthesis of triangular gold nanoprism using lemon grass leaf extract showed large size of nanoprisms ranging from — nm length and truncated vertices [14].

This microbial synthesis of GNPs could be explained by the fact that microorganisms often face metal ions or metals in their environment and thereby developed mechanism to detoxify metals by changing the redox states of metal ions and or intracellular precipitation of metals forming a basis for synthesis of nanoparticles [32].

This report provides for the first time rapid novel environment friendly cell surface based ot of stable gold nanoprisms at room temperature using Penicillium citrinum MTCC biomass. Sample images were processed using ProScan image processing software.

FTIR spectroscopy data also supported the fact that biomolecules were present in the surface. In our study, GNPs formed a single population in the size range of 10 — 40 nm. Thus a visual inspection was routinely performed. The strain nanoprism subcultured on potato dextrose agar PDA.

The ring structure of SAED biokogical is a characteristic of polycrystalline gold Figure 1 B represents GNPs solution collected from mycelial surfaces sythesis sequential sonication and filtration. UV-Visible spectra of gold nanoparticles by bio-reduction of Chloroauric acid in aqueous solution was recorded in Jasco UV-Visible spectrophotometer V operated at a resolution of 1 nm in absorption mode.

Formation of gold nanoparticles resulted in a sharp colour change from yellow to dark pinkish. FTIR spectra of gold nanoparticles. Metal nanoparticles are produced mainly by means of chemical processes involving chemical aggressive reducing agents e.

The biomolecules present in the cell surface of Penicillum citrinum may perform three important functions: Then the biomass was harvested by sieving through a plastic filter and washed several times with double distilled water to remove any traces of media components. Some rod shaped, triangular, cubical, very small circular 3 — 4 nm diameter and rectangular GNPs were present in smaller amounts Figures A and B.

For FTIR spectrum analysis the GNPs were centrifuged at 10, rpm for 10 minutes to remove free proteins or other compounds present in the solution.

A Formation of GNPs on mycelial biomass resulting purple color. Due to symmetric face centered cubic fcc lattice of gold nanoparticles, the formation of anisotropic structures like cube, prism, star, branched nanoparticles require a selective capping agent during growth To investigate the physical nature of gold nanoparticles transmission electron microscopic analysis was done.

B Color of GNPs solution. Thus this is quite exciting for us to synthesize gold nanoprism for first time using biological approach. TOP Related.

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BIOLOGICAL SYNTHESIS OF TRIANGULAR GOLD NANOPRISMS PDF

Zolokus Biological synthesis of triangular gold nanoprisms. By conventional chemical and physical methods nanocubes [22], nanorods [23], nanowires [24], nanoplates [25] can be synthesized but that require high environmental cost. Free amine, carboxyl or phosphate groups present in the protein can interact with gold nanoparticles [32,37] and possibly involved in stabilization of GNPs. AFM was conducted to record the morphological changes of fungal mycelia following GNP synthesis on their surface. The selected area electron diffraction pattern SAED of gold nanoprism Figure 4 B indicated that the entire nanoparticle was nanocrystalline in nature [31]. There are other reports which showed the intracellular synthesis of spherical GNPs in live Alfaalfa plants [12], truncated triangular and spherical GNPs from sun dried Cinamonum camphora leaf extract [13], triangular gold nanoprism from lemon grass extract [14], hexagonal, prism and spherical shaped GNPs using plant surfactants [15], triangular gold nano plates using algal solutions of Chlorella vulgaris [16] and Sargassum sp.

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